africa green monkey kidney cells vero  (BioResource International Inc)

Journal: Oncotarget

Article Title: Oncolytic virotherapy with SOCS3 enhances viral replicative potency and oncolysis for gastric cancer

doi: 10.18632/oncotarget.27873

Figure Lengend Snippet: ( A ) BAC plasmids were digested with Hind III. The digested BAC plasmids were electrophoresed, T-BAC (left) and Cre-recombinant BAC plasmid, T-BAC/SV-SOCS3 (right). ( B ) Western blotting for Vero cells and MKN1 cells infected with T-SOCS3 (at MOI of 0.01) with an anti-SOCS3 antibody.

Article Snippet: Africa green monkey kidney cells (Vero) and human gastric cancer cell lines (MKN1, MKN28, and MKN74) were made a purchase from the RIKEN BioResource Center (Tsukuba, Japan).

Techniques: Recombinant, Plasmid Preparation, Western Blot, Infection

Journal: Oncotarget

Article Title: Oncolytic virotherapy with SOCS3 enhances viral replicative potency and oncolysis for gastric cancer

doi: 10.18632/oncotarget.27873

Figure Lengend Snippet: ( A ) Vero cells and MKN1 cells were seeded into 6 well plates at a density of 5 × 10 5 per well. Each well was infected through either T-01 or T-SOCS3 at MOI of 0.01 (for MKN1 cells, at MOI of 0.1) and the viral replication was determined. The viral titer of T-SOCS3 had an approximately 20-fold enhanced ability to replicate relative to T-01 in MKN1 cells ( ** p < 0.01). ( B ) MKN1 human gastric cancer cells were seeded in 10-cm dish at 2 × 10 6 cells per dish and incubated at 37°C. After 24 hr of incubation, cells were infected through PBS (–) and T-01 (MOI of 0.1) and incubated further at 39.5°C for 20 hr and harvested. Proteins (30 μg) were applied to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membrane, and blotted for 2 hr with monoclonal mouse anti-phosphorylated-STAT3 (pSTAT3) antibody or an hour with mouse anti-b-actin antibody (diluted 1:2000, Sigma).

Article Snippet: Africa green monkey kidney cells (Vero) and human gastric cancer cell lines (MKN1, MKN28, and MKN74) were made a purchase from the RIKEN BioResource Center (Tsukuba, Japan).

Techniques: Infection, Incubation, Polyacrylamide Gel Electrophoresis, SDS Page, Membrane

Journal: Pathogens and Disease

Article Title: Intravenous adenovirus expressing a multi-specific, single-domain antibody neutralizing TcdA and TcdB protects mice from Clostridium difficile infection

doi: 10.1093/femspd/ftw078

Figure Lengend Snippet: ABA purification and function. ABA was purified from culture supernatants of Vero cells infected with rAd-ABA. (a) The purified ABA exhibited a single dominant band at 66 kDa on a SDS-PAGE gel. (b) Dot blotting showed binding of ABA to TcdA and TcdB. Serially diluted TcdA and TcdB proteins were spotted on nitrocellulose paper. The paper was then blocked with milk before adding ABA at 1 μg ml−1 final concentration. The binding of ABA to both TcdA and TcdB was determined by an HRP-conjugated anti-E-tag antibody.

Article Snippet: The control GFP plasmid construction and rAd-GFP preparation were carried out the same way except that the ABA gene was replaced with the GFP gene in the constructs. rAd-ABA infection of cultured cell lines and purification of ABA from culture supernatants rAd-ABA virions were used to infect Africa green monkey kidney epithelial cell line Vero cells (ATCC, Manassas, VA).

Techniques: Purification, Infection, SDS Page, Binding Assay, Concentration Assay